Metadata-Version: 2.1
Name: porerefiner
Version: 0.8.2
Summary: To help you manage your pores
Home-page: https://github.com/CFSAN-Biostatistics/porerefiner
Author: Justin Payne
Author-email: justin.payne@fda.hhs.gov
License: MIT license
Keywords: porerefiner
Platform: UNKNOWN
Classifier: Development Status :: 2 - Pre-Alpha
Classifier: Intended Audience :: Developers
Classifier: License :: OSI Approved :: MIT License
Classifier: Natural Language :: English
Classifier: Programming Language :: Python :: 3.7
Requires-Python: >=3.7
Requires-Dist: Click (>=7.0)
Requires-Dist: flask (>=1.1.1)
Requires-Dist: peewee (>=3.11.2)
Requires-Dist: gunicorn (>=19.9.0)
Requires-Dist: watchdog (>=0.9.0)
Requires-Dist: hachiko (>=0.2.0)
Requires-Dist: aiohttp (>=3.6.1)
Requires-Dist: namesgenerator (>=0.3)
Requires-Dist: python-daemon (>=2.2.3)
Requires-Dist: protobuf (>=3.10.0)
Requires-Dist: grpclib (>=0.3.0)
Requires-Dist: tabulate (>=0.8.5)
Requires-Dist: asyncssh (>=2.0.1)
Requires-Dist: setproctitle (>=1.1.10)
Requires-Dist: pyyaml (>=5.3)

===========
PoreRefiner
===========


.. image:: https://img.shields.io/pypi/v/porerefiner.svg
        :target: https://pypi.python.org/pypi/porerefiner

.. image:: https://img.shields.io/travis/crashfrog/porerefiner.svg
        :target: https://travis-ci.org/crashfrog/porerefiner

.. image:: https://readthedocs.org/projects/porerefiner/badge/?version=latest
        :target: https://porerefiner.readthedocs.io/en/latest/?badge=latest
        :alt: Documentation Status




To help you manage your pores


* Free software: MIT license
* Documentation: https://porerefiner.readthedocs.io.


Introduction
------------

PoreRefiner is a software tool to watch Nanopore runs in progress and attach sample information to them, as well as provide an interface for integration with LIMS services and other online systems. It supports both push and pull modalities for data exchange with those systems - push, via a series of configurable notifiers, and pull, via a simple Flask webservice and a Protobuf RPC service. It also includes a command-line interface for working with the run database.

Installation
------------

PoreRefiner is available as a Python package:

::

    pip install porerefiner

Copy the files ``porerefiner.service`` and ``porerefiner.app.service`` from the package to systemd:

::

    cp /usr/local/lib/python3.7/dist-packages/porerefiner.service /lib/systemd/system
    cp /usr/local/lib/python3.7/dist-packages/porerefiner.app.service /lib/systemd/system
    systemctl enable porerefiner.service
    systemctl enable porerefiner.app.service

Once the package is installed, ``porerefinerd`` and ``prfr`` should be on your path. You can use ``porerefinerd init`` to set up the config file for the porerefiner service, it will prompt you for the save locations of the database, the local socket, nanopore's output directory, and where the config file should be saved:

::

    $ porerefinerd init
    create PoreRefiner config at /etc/porerefiner/config.yaml? [y/N]: y
    location of porerefiner RPC socket? [/etc/porerefiner/porerefiner.sock]:
    location of database? [/etc/porerefiner/database.db]:
    nanopore data output location?: /data
    export POREREFINER_CONFIG="/etc/porerefiner/config.yaml"

To the end of the ``config.yaml`` (section ``submitters``) add:

::

    submitters:
    - class: HpcSubmitter
      config:
        login_host: login1-raven2.fda.gov
        username: nanopore
        private_key_path: /root/.ssh/nanopore
        known_hosts_path: /root/.ssh/known_hosts
        scheduler: uge
        queue: service.q
      jobs:
      - class: FdaRunJob
        config:
          command: module load nanopore-lims/0.1.0 && nanopore_HPC {remote_json} &
          platform: GridION sequence
          closure_status_recipients:
          - justin.payne@fda.hhs.gov
          import_ready_recipients:
          - justin.payne@fda.hhs.gov

This configures PoreRefiner for the FDA Raven integration. Then you can start the porerefiner services:

::

    systemctl start porerefiner.service
    systemctl start porerefiner.app.service

If you wish to enable the PoreRefiner web interface, you should ensure that port 8844 is reachable from remote hosts.

Using this software
-------------------

``prfr`` is the end-user client; Minion users should use this tool to monitor runs in progress, load sample sheets, and tag runs and samples.

::

    $ prfr --help
    Usage: prfr [OPTIONS] COMMAND [ARGS]...

      Command line interface for PoreRefiner, a Nanopore run manager.

    Options:
    --help  Show this message and exit.

    Commands:
    info      Return information about a run, historical or in progress.
    load      Load a sample sheet to be attached to a run, or to the next run...
    ps        Show runs in progress, or every tracked run (--all), or with a...
    tag       Add one or more tags to a run.
    template  Write a sample sheet template to STDOUT.
    untag     Remove one or more tags from a run.


Administration
--------------

When the PoreRefiner service is stopped, it has a number of administrative functions:

::

    $ porerefinerd --help
    Usage: porerefiner.py [OPTIONS] COMMAND [ARGS]...

    Options:
    --help  Show this message and exit.

    Commands:
    init    Find the Nanopore output directory and create the config file.
    list    List job system stuff.
    reset   Utility function to reset various state.
    start   Start the PoreRefiner service.
    verify  Run various checks.

::

    $ porerefinerd init --help
    Usage: porerefiner.py init [OPTIONS]

    Find the Nanopore output directory and create the config file.

    Options:
    --config TEXT
    --nanopore_dir TEXT
    --help               Show this message and exit.

::

    $ porerefinerd list --help
    Usage: porerefiner.py list [OPTIONS] COMMAND [ARGS]...

    List job system stuff.

    Options:
    --help  Show this message and exit.

    Commands:
    jobs        List the configurable and configured jobs.
    notifiers   List the configurable and configured notifiers.
    submitters  List the configureable and configured submitters.

::

    $ porerefinerd reset --help
    Usage: porerefiner.py reset [OPTIONS] COMMAND [ARGS]...

    Utility function to reset various state.

    Options:
    --help  Show this message and exit.

    Commands:
    config        Reset config to defaults.
    database      Reset database to empty state.
    jobs          Reset all jobs to a particular status.
    runs          Reset all runs to in-progress status.
    samplesheets  Clear samplesheets that aren't attached to any run.

::

    $ porerefinerd verify --help
    Usage: porerefiner.py verify [OPTIONS] COMMAND [ARGS]...

    Run various checks.

    Options:
    --help  Show this message and exit.

    Commands:
    notifiers   Verify notifiers by sending notifications.
    submitters  Verify configuration of job submitters by running their tests.


Features
--------

Automatic detection of runs in progress

Sample sheet and sample tracking through the flowcell/run context, and beyond

Schedule automatic analysis of runs and files in AWS or your HPC

How it works
------------

PoreRefiner uses fsevents to detect filesystem events during a Nanopore run, including the creating of new directories in the Nanopore output folder. Flowcells, runs, and run files can be detected this way. PoreRefiner will update a SQLite database with run information, including what it's able to pull out of Minknow.

If all of the files of a run have not been modified in an hour, PoreRefiner will mark a completion time for that run. If any of the files in a run have not been modified in an hour, they may be picked up by the Job runner for some subsequent processing.

PoreRefiner presents many interfaces to address integration challenges:

A CLI interface for both human use and simple scripting

A simple HTTP service for communication with LIMS and other services

A Protobuf-RPC service for inter-process communication (Protobuf bindings are available in Python, C, JavaScript, Java, and many other languages)

Credits
-------

This package was created with Cookiecutter_ and the `audreyr/cookiecutter-pypackage`_ project template.

.. _Cookiecutter: https://github.com/audreyr/cookiecutter
.. _`audreyr/cookiecutter-pypackage`: https://github.com/audreyr/cookiecutter-pypackage


=======
History
=======

0.8.2 (2020-03-12)
------------------

* Fixed tests, general bugfixes

0.8.1 (2020-03-11)
------------------

* Improved service files.

0.8.0 (2020-03-09)
------------------

* First release on PyPI.


