LOCUS       AJ001614                3128 bp    DNA     circular BCT 14-NOV-2006
DEFINITION  Escherichia coli plasmid pCAPs.
ACCESSION   AJ001614
VERSION     AJ001614.1  GI:2769261
KEYWORDS    ampicillin resistance; beta-lactamase; bla gene; catabolite gene
            activator protein; crp gene; cyclic amp receptor protein.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Schlieper,D., von Wilcken-Bergmann,B., Schmidt,M., Sobek,H. and
            Muller-Hill,B.
  TITLE     A positive selection vector for cloning of long polymerase chain
            reaction fragments based on a lethal mutant of the crp gene of
            Escherichia coli
  JOURNAL   Anal. Biochem. 257 (2), 203-209 (1998)
   PUBMED   9514792
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Schlieper,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-1997) Schlieper D., Institute of Genetics,
            University of Cologne, Weyertal 121, Cologne, 50931, GERMANY
FEATURES             Location/Qualifiers
     source          1..3128
                     /db_xref="taxon:562"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli"
                     /plasmid="pCAPs"
     gene            complement(315..947)
                     /gene="crp"
     CDS             complement(315..947)
                     /product="catabolite gene activator protein"
                     /codon_start=1
                     /transl_table=11
                     /note="artificially mutated crp; cyclic AMP receptor
                     protein"
                     /db_xref="GI:2769262"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVK
                     GSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKKFR
                     QLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVSGRIAQTLLNLAKQPDAMTHPDG
                     MQIKITRQEIGQIVGCSRQTVGRILKMLEDQNLISAHGKTIVVYGTR"
                     /gene="crp"
                     /protein_id="CAA04867.1"
     rep_origin      1313
                     /direction=BOTH
     gene            complement(2072..2932)
                     /gene="bla"
     CDS             complement(2072..2932)
                     /product="beta-lactamase"
                     /codon_start=1
                     /transl_table=11
                     /db_xref="GI:2769263"
                     /db_xref="GOA:Q79DR3"
                     /db_xref="HSSP:P62593"
                     /db_xref="InterPro:IPR000871"
                     /db_xref="InterPro:IPR001466"
                     /db_xref="InterPro:IPR012338"
                     /db_xref="UniProtKB/TrEMBL:Q79DR3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /gene="bla"
                     /protein_id="CAA04868.1"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatagatc ctgaggatcg gggtgataaa tcagtctgcg ccacatcggg ggaaacaaaa
      241 tggcgcgaga tctaaaaaaa aaggctccaa aaggagcctt tcgcgctacc aggtaacgcg
      301 ccactccgac gggattaacg agtgccgtaa acgacgatgg ttttaccgtg tgcggagatc
      361 aggttctgat cctcgagcat cttaagaatt cgtcccacgg tttgtctaga gcagccgaca
      421 atctggccaa tttcctgacg ggtaattttg atttgcatgc cgtccgggtg agtcatagcg
      481 tctggttgtt ttgccagatt cagcagagtc tgtgcaatgc ggccgctgac gtcgaggaac
      541 gccaggttgc ccactttctc actagtgacc tgcagccggc gcgccatctg tgcagacaaa
      601 cgcatcagga tatccggatt tacctgaatc aattggcgaa attttttgta cgaaatttca
      661 gccacttcac aggcggtttt cgcacgtacc catgcgctac gttcctggcc ctcttcaaac
      721 aggcccagtt cgccaataaa atcaccctga ttcagatagg agaggatcat ttctttaccc
      781 tcttcgtctt tgatcagcac tgccacagag cctttaacga tgtagtacag cgtttccgct
      841 ttttcaccct ggtgaataag cgtgctcttg gatgggtact tatgaatgtg gcaatgagac
      901 aagaaccatt cgagagtagg atccgtttga ggtttaccaa gtaccataag atccttaaat
      961 ttttattatc tagctagatg ataatattat atcaagaatt gtacctgaaa gcaaataaat
     1021 tttttatctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     1081 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     1141 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1201 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1261 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1321 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1381 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1441 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1501 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1561 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1621 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1681 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1741 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1801 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1861 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1921 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1981 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2041 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2101 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2161 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2221 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2281 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2341 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2401 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2461 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2521 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2581 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2641 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     2701 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2761 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     2821 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     2881 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     2941 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3001 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3061 ctgctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     3121 ctttcgtc
//