Metadata-Version: 2.1
Name: uceasy
Version: 0.3.1
Summary: Wrapper for the Phyluce phylogenomics software package
Home-page: https://uceasy.github.io
License: MIT
Keywords: uce,phyluce,ultraconserved elements
Author: Caio Raposo
Author-email: caioraposo@protonmail.com
Requires-Python: >=3.7,<4.0
Classifier: License :: OSI Approved :: MIT License
Classifier: Programming Language :: Python :: 3
Classifier: Programming Language :: Python :: 3.7
Classifier: Programming Language :: Python :: 3.8
Classifier: Programming Language :: Python :: 3.9
Classifier: Programming Language :: Python :: 3.10
Classifier: Programming Language :: Python :: 3.11
Requires-Dist: click (>=8.0,<9.0)
Project-URL: Repository, https://github.com/uceasy/uceasy
Description-Content-Type: text/markdown

<p>
    <img src="docs/img/uceasy_logo.png" height="200px">

</p>

[![Tests](https://github.com/uceasy/uceasy/workflows/Tests/badge.svg)](https://github.com/uceasy/uceasy/actions?workflow=Tests)
[![Quality Gate Status](https://sonarcloud.io/api/project_badges/measure?project=uceasy_uceasy&metric=alert_status)](https://sonarcloud.io/dashboard?id=uceasy_uceasy)
[![DOI](https://zenodo.org/badge/203415455.svg)](https://zenodo.org/badge/latestdoi/203415455)

# UCEasy: A software package for automating and simplifying the analysis of ultraconserved elements (UCEs)


__UCEasy__ is a Python wrapper that standardizes, automates, and simplifies the following [PHYLUCE](https://phyluce.readthedocs.io/en/latest) tasks: quality control of raw reads, assembly, alignment and UCE loci extraction.


Although the PHYLUCE pipeline is robust and well-established, it requires the execution of many command line scripts from heterogeneous tools, which can be quite challenging for scientists without some training in bioinformatics. 

For more information check out our [Wiki](https://github.com/uceasy/uceasy/wiki).
## Installation Guide
### Dependencies
* Python ^3.7
* PHYLUCE 1.6.* (1.7 not supported yet)

Install the package from [PyPI](https://pypi.org/project/uceasy/):
```
$ pip install uceasy
```
Then, make sure you have a working installation of PHYLUCE, check out the installation guide at [PHYLUCE's documentation](https://phyluce.readthedocs.io/en/latest/installation.html).


## Workflow
The operations [Trim](https://github.com/uceasy/uceasy/wiki/Trim), [Assemble](https://github.com/uceasy/uceasy/wiki/Assemble) and [Align](https://github.com/uceasy/uceasy/wiki/Align) represent the following workflow. The following image demonstrates how the PHYLUCE workflow is abstracted by UCEasy.

<p>
    <img src="docs/img/phyluce-uceasy-comparison.png" height="500px">

</p>

The colored boxes are UCEasy CLI commands.
```
$ uceasy trim
$ uceasy assemble
$ uceasy align
```
Explore the options for these commands with the `--help` flag.<br>

For a guide of how to use UCEasy see: [Tutorial](https://github.com/uceasy/uceasy/wiki/Tutorial).


## Acknowledgements

We thank the following institutions, which contributed to ensuring the success of our work:

Ministério da Ciência, Tecnologia, Inovação e Comunicação (MCTIC)

Museu Paraense Emílio Goeldi (MPEG)

Instituto Nacional de Pesquisas da Amazônia (INPA)

Centro Universitário do Estado do Pará (CESUPA)

## Funding

 This research was supported  by Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq (fellowships 149985/2018-5; 129954/2018-7). Romina Batista was supported by a postdoctoral fellowship granted from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES-INPA proc. 88887477562/2020-00).


## Authors

 Caio Vinícius Raposo Ribeiro<br>
 Lucas Peres Oliveira<br>
 Romina Batista<br>
 Marcos Paulo Alves de Sousa


 ## Contact

Dr. Marcos Paulo Alves de Sousa (Project leader)

_Email: **msousa@museu-goeldi.br**_<br>
_Laboratório de Biologia Molecular-LBM_<br>
_Grupo de pesquisa em Bioinformática e Informática para Biodiversidade (BioInfo)_<br>
_Museu Paraense Emílio Goeldi_<br>
_Av. Perimetral 1901. CEP 66077- 530. Belém, Pará, Brazil._

